Human protein Z (PZ) is a single chain, vitamin K-dependent plasma protein. Analogous with the majority of the coagulation proteins, protein Z is synthesized in the liver. The mature protein contains 360 amino acids. Based on amino acid sequence homology the domain structure is similar to that of other vitamin K-dependent zymogens which include factor VII, factor IX, factor X, and protein C. The N-terminal region contains a carboxyglutamic acid (Gla) domain important in its phospholipid membrane binding ability. Following the N-terminal Gla domain are two EGF domains and a region which connects to a catalytic-like domain. The C-terminal region has been shown to lack the ''typical serine protease activation site as well as the His and Ser residues from the catalytic triad. Protease activity has not been detected in either the full-length protein or cleavage products of protein Z. Functionally protein Z has been shown to be a direct requirement for the binding of thrombin to endothelial phospholipids. Protein Z also serves as a cofactor for the inhibition of coagulation factor Xa by a plasma serpin called protein Z-dependent protease inhibitor (ZPI). Inhibition is dependent upon complex formation between factor Xa-PZ-ZPI on the phospholipid surface. Localization: Plasma Mode of Action: Required for the binding of thrombin to phospholipid surfaces and for PZ dependant inhibition of factor Xa. Extinction Coefficient (1%, 1cm, 280nm): 12.0 Plasma Concentration: ~2.7ug/ml Structure: Single chain, (Mr=62,000), displays structural similarity to other vitamin K-dependent coagulation factors based on sequence homology, most notably factor X Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
62000
Purity:
98% SDS-PAGE. Human protein Z is prepared from fresh frozen plasma similar to the procedure described by Broze and Miletich.
Form:
Supplied as a liquid in 50% glycerol, dH2O.
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