Psp5II (PpuMI)

Product Overview

• Article Name: Psp5II (PpuMI)
• Biozol Catalog Number: USB-P9177
• Supplier Catalog Number: P9177
• Alternative Catalog Number: USB-P9177-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

T 5-Pu G G A C C Py-3 3-Py C C T G G Pu-5 A Concentration: 10u/ul Source: Pseudomonas fluorescens RFL5 Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.1% Triton X-100, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Psp5II. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Psp5II, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.01uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected. Methylation Effects: Psp5II does not cut PuGG(A/T)Cm5CPy. Blocked by overlapping Dcm methylation. Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: Cfr13I, CpoI, Eco47I, SanDI Number of Recognition Sites in DNA: Lambda: 3 PhiX174: 0 M13mp18/19: 0 pBR322: 2 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 pBluescriptIIKS(-/+): 0 pBluescriptIISK(-/+): 0 pACYC177: 0 pACYC184: 2 3 0 0 2 0 0 0 0 0 0 2 Unit Definition. One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-CpoI (RsrII) fragments in 1 hour at 37C in 50ul of assay buffer.