PstI 10u/ul

Product Overview

• Article Name: PstI 10u/ul
• Biozol Catalog Number: USB-P9180
• Supplier Catalog Number: P9180
• Alternative Catalog Number: USB-P9180-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-C T G C A G-3 3-G A C G T C-5 Concentration: 10 units/ul (see P9180-05 for 50u/ul) Source: Providencia stuarti Supplied With: R1625: Restriction Enzyme Buffer A, 10X R1625-03: Restriction Enzyme Buffer D, 10X Concentration: 10u/ul Incubation Temperature: 37C Unit Definition: One unit is defined as the amount of PstI required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer. Diluent Buffer: 10mM Tris-HCl pH 7.4,100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. For longer periods, the Storage Buffer should be used. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl pH 7.4, 200mM sodium chloride, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA, 50% glycerol. Enzyme Properties: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with PstI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with PstI, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.25uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of PstI for 4 hours. Blue/White Cloning Assay: pUC57 was digested at a unique site with 10 units of PstI for 16 hours. After religation and transformation < 1% of white colonies were detected. Methylation Effects: Dam/Dcm/CpG/EcoKI/EcoBI: never overlaps - no effect Stability During Prolonged Incubation: A minimum of 0.2 units of PstI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: PstI is not inactivated by incubation at 80C for 20 min. Digestion of Agarose-embedded DNA: A minimum of 5 units of PstI is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: Alw21I, BseSI, Mph1103I, SdaI, SduI Number of Recognition Sites in DNA: Lambda: 28 PhiX174: 1 pBR322: 1 pUC57: 1 pUC18/19: 1 pTZ19R/U: 1 M13mp18/19: 1 Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.