PsuI (BstYI)

Product Overview

• Article Name: PsuI (BstYI)
• Biozol Catalog Number: USB-P9181
• Supplier Catalog Number: P9181
• Alternative Catalog Number: USB-P9181-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-RG A T C Y-3 3-Y C T A GR-5 Source: Pseudomonas stutzeri Mck28-pH52 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of PsuI required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of recommended reaction buffer. Form: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Supplied with R1625-01 Restriction Enzyme Buffer B, 10X: Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 0.1mg/ml BSA. Incubation Temperature: 37C Thermal Inactivation: PsuI is inactivated by incubation at 80C for 20 minutes. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after a 80-fold overdigestion with PsuI (5u/ug lambda DNA x 16 hours). Ligation and Recleavage (L/R) Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of single-stranded or double-stranded labeled oligonucleotides occurred during incubation with 10 units of PsuI for 4 hours. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol Enzyme Properties: Methylation Effects: Dam, Completely overlaps - no effect Dcm: May overlap - no effect CpG: May overlap - no effect EcoKI: Never overlaps - no effect EcoBI: May overlap - effect not determined Stability during Prolonged Incubation: A minimum of 0.5units of PsuI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Compatible Ends: BamHI, BclI, Bsp143I, MboI, BglII Number of Recognition Sites in DNA: Lambda: 21 PhiX174: 0 pBR322: 8 pUC57: 7 pUC18/19: 7 pTZ19R/U: 7 M13mp18/19: 3 Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.