PKLR, Control Peptide (Pyruvate Kinase Isozymes R/L, Pyruvate Kinase 1, R-type/L-type Pyruvate Kinase, Red Cell/Liver Pyruvate Kinase, PK1, PKL)
Biozol Catalog Number:
USB-P9545-03E
Supplier Catalog Number:
P9545-03E
Alternative Catalog Number:
USB-P9545-03E-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Control Peptide for P9545-03D. Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, yielding a pyruvate molecule. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues Highly expressed in liver and RBCs. The PKLR gene codes for both the liver and red blood cell isozymes. The PKM2 gene, located on chromosome 15q22, encodes 2 muscle-specific isoforms, M1 and M2. The PK enzyme functions as a homotetramer. Studies with the rat enzyme suggested that the red cell type is longer than the liver type in the 5-prime terminal end, but the rest of the sequence is very similar. deduced protein sequence was similar to that of L-type PK, with an additional 31aa at the N-terminus. Additional studies demonstrated that the difference between the L- and R-type pyruvate kinases is due to tissue-specific mRNAs, likely resulting from differential processing of a common nuclear RNA precursor. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Source: Synthetic peptide corresponding to 19aa peptide of mouse PKLR (accession P53657), N-terminal (KLH). Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-30ug control peptide per 1ug of aff pure IgG or 1ul antiserum Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
