Human RANK (receptor activator of NF-KappaB), a soluble 20kD protein, comprising the full-length of the TNF-like extracellular domain of RANKL, is a member of the TNFR family, identified as a dentritic cell membrane protein. Recombinant protein corresponding to human sRANKL, a 20kD polypeptide comprising the TNF-homologous region of RANKL (176 amino acid residues), expressed in E. coli Biological Activity: Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-kB Luc reporter cells. Amino Acid Sequence: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
98% by SDS-PAGE and HPLC analysis. Endotoxin: 0.1ng/ug (1EU/ug)
Form:
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5. No preservatives added. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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