RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant mouse sRANKL is a 19.4kD polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues), expressed in E. coli. Biological Activity: Measured by its dose-dependent ability to induce reporter gene in HT-29 NF-kB Luc reporter cells Amino Acid Sequence: PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Do not vortex. For long term storage add a carrier protein (0.1% BSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
19.4
Purity:
98% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug (1EU/ug).
Form:
Supplied as a lyophilized powder from 5mM sodium phosphate, pH 7.6, 75mM sodium chloride. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex
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