RANTES, Recombinant, Human (Regulated on Activation, Normal T-cell Expressed and Secreted)

Product Overview

• Article Name: RANTES, Recombinant, Human (Regulated on Activation, Normal T-cell Expressed and Secreted)
• Biozol Catalog Number: USB-R1100-05
• Supplier Catalog Number: R1100-05
• Alternative Catalog Number: USB-R1100-05-5,USB-R1100-05-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant human RANTES is a 7.8kD protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC-chemokines. Amino Acid Sequence: SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS Endotoxin: 0.1ng/ug (1EU/ug) Biological Activity: Determined by its ability to chemoattract human blood monocytes using a concentration range of 1-10ng/ml. Responding Cells (partial list): Monocytes Concentration Range for most in vitro applications: 0.2-20ng/ml Reconstitution: Lyophilized RANTES is soluble in water and most aqueous buffers. Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile dH2O. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA

Product Properties

• Molecular Weight: 7.8
• Purity: 98% by SDS-PAGE and HPLC analyses.
• Form: Supplied as a lyophilized powder without additives.