RANTES, Recombinant, Rat (Regulated on Activation, Normal T-cell Expressed and Secreted, C-C Motif Chemokine 5, Ccl5, Scya5, CCL5)

Product Overview

• Article Name: RANTES, Recombinant, Rat (Regulated on Activation, Normal T-cell Expressed and Secreted, C-C Motif Chemokine 5, Ccl5, Scya5, CCL5)
• Biozol Catalog Number: USB-R1100-10
• Supplier Catalog Number: R1100-10
• Alternative Catalog Number: USB-R1100-10-5,USB-R1100-10-20,USB-R1100-10-50,USB-R1100-10-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma. Recombinant protein corresponding to rat RANTES, a 7.8kD protein containing 68 amino acid residues, including the four highly conserved cysteine residues present in the CC chemokines., expressed in E. coli. Swiss/Uniprot: P50231 Biological Activity: Rat Rantes produces chemotaxis over a wide concentration range in assay using rat peritoneal macrophages. Significant chemotaxis was achieved using a concentration of 50-100ng/ml. Responding Cells (partial list): Monocytes, T cells, others Amino Acid Sequence: SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 7.9
• UniProt: P50231
• Purity: 98% (SDS-PAGE and HPLC analysis). Endotoxin: 0.1ng/ug
• Form: Supplied as a lyophilized powder with no additives. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex. For long term add protein carrier. It is recommended that further dilutions be made into other aqueous buffers.