RAP, Recombinant, Rat (Receptor Associated Protein)
Biozol Catalog Number:
USB-R1115-15
Supplier Catalog Number:
R1115-15
Alternative Catalog Number:
USB-R1115-15-100,USB-R1115-15-250
Manufacturer:
US Biological
Category:
Molekularbiologie
Rat his:RAP:c-myc fusion protein RAP binds with high affinity to certain members of the low-density lipoprotein (LDL) receptor family such as LDL receptor-related protein (LRP), gp330/megalin, and the very low-density lipoprotein (VLDL) receptor and, once bound to these receptors, antagonizes their ligand binding ability. Despite the presence of a putative signal sequence on RAP, this molecule is not secreted but remains cell-associated. RAP is contained primarily within the endoplasmic reticulum and Golgi compartments, with only a trace found at the cell surface and within the endosomal compartments. Genetic deletion of RAP in mice has no effect on LRP mRNA levels but does lead to incomplete processing of LRP in the brain and liver resulting in decreased LRP antigen levels in these organs. Molecular Weight: 40kD (SDS gel electrophoresis). Calculated MW from sequence data of the mature protein is 38.862kD (Source: NCBI sequence viewer) Applications: Suitable for use in Immunoblot, Receptor-binding studies and as protein standard in 1D and 2D SDS gel electrophoresis. Recommended Dilutions: Note: Ligand binding to RAP is Ca2+ dependent. Lipid receptors can be released from RAP by a buffer containing 10mM EDTA. Buffers containing phosphate should be avoided for it forms precipitates with Ca2+. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
40
Purity:
95% (SDS-PAGE)
Form:
Supplied as a lyophilized powder from TBS, pH 7.5, 0.1% BSA, and 0.09% sodium azide. Reconstitute with 100ul dH2O.
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