RAP1A, aa1-181, Recombinant, Human (Ras-related Protein Rap-1A, C21KG, G-22K, Krev-1, Ras-related Protein Krev-1, KREV1)
Biozol Catalog Number:
USB-R1120-19
Supplier Catalog Number:
R1120-19
Alternative Catalog Number:
USB-R1120-19-50,USB-R1120-19-250
Manufacturer:
US Biological
Category:
Molekularbiologie
Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% aa identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st aa: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Source: Recombinant corresponding to aa1-181 of human HRAS, 6x His tagged, expressed in E.coli. AA Sequences: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C Molecular Weight: 22.8kD (201aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
