Each kit contains 20 individual 10-mer primers (supplied at a minimum amount of 50 nmole per primer). These primers are suitable for use in genetic mapping and DNA fingerprinting. In the RAPD technique, a single 10mer of arbitrary sequence is used as a primer in PCR to amplify genomic DNA where the sequence of the DNA is completely unknown. Genomic DNA from different individuals gives different PCR products, allowing the identification of DNA polymorphisms that can be used to identify different individuals or as genetic markers. The number of PCR products generated from each genomic DNA sample depends on the primer sequence, the genomic DNA sequence, and the genome size. Assuming that the priming sites of a 10mer RAPD primer are randomly distributed throughout a genome, the theoretical number of PCR products is approximately 2.5x10e9 xG, where G is the size of the haploid genome in base pairs. Using this calculation, a haploid genome of 2x10e9bp, for example, is expected to give about 5 PCR products. This prediction is in close agreement with experimental results. Kit Components: 01 CAC ACC GTG T 11 ACC GTG CCG T 02 TGA CCA GGC A 12 GTC CTC GTG T 03 ACG GTT CCA C 13 CAC GGA CCG A 04 GTC TTG GGC A 14 TCG CAG CGT T 05 GTC ACC TGC T 15 CTG CAA TGG G 06 GGC GCG TTA G 16 AAC CCT TCC C 07 GAC GAG CAG G 17 AGT TCC GCG A 08 GGC TGC CAG T 18 GTT GCG CAG T 09 TGG AGT CCC C 19 TGA CAG CCC C 10 CCC GTC TAC C 20 TCT GCC TGG A Storage and Stability: Store at -20C. Lyophilized powder is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Salt-free
Form:
Each primer is supplied as a lyophilized powder. Salt Free. Reconstitute with sterile dH2O, TE or Tris pH 7.0.
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