Source: E.coli Unit Definition: One unit of the enzyme incorporates I nmole dTTP into acid-precipitable material in 10 minutes at 37C, using poly (A): oligo dT as a template:primer. Quality Assurance: Free of endonucleases and exonucleases Components: 1. M-MLV Reverse Transcriptase (200units/ul) 2. 5X reaction buffer Quality Control: Nonspecific Endonuclease/Nickase Activity: None detected after 14-16 hour incubation of 100units of enzyme with 300ng of supercoiled plasmid DNA, analyzed on agarose gel. Exonuclease Activity: None detected after 14-16 hour incubation of 100units of enzyme with 40ng of 32P-labeled Sau3A fragments of pUC19, analyzed by PAGE. Protease Activity: None detected after 14-16 hour incubation of 1200units of enzyme with 1ug of protease substrate, analyzed by fluorescence. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form:
Supplied as a liquid in (5X) 250mM Tris HCl, pH 8.3, 375mM KCl, 15mM MgCl2, 50mM DTT.
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