Ribonuclease Inhibitor, Placenta (RI, RNase Inhibitor)

Product Overview

• Article Name: Ribonuclease Inhibitor, Placenta (RI, RNase Inhibitor)
• Biozol Catalog Number: USB-R2025A
• Supplier Catalog Number: R2025A
• Alternative Catalog Number: USB-R2025A-5000
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Storage Buffer: 20mM HEPES-KOH (pH 7.5), 50mM KCl, 5mM DTT and 50% glycerol. Unit Definition: One unit is the amount of enzyme required to inhibit by 50% the activity of 5ng of RNase A at 25C (This inhibitor activity is determined by its ability to inhibit hydrolysis of cyclic 2, 3-CMP by RNase A). Quality Control: Nuclease activity was not detected in any of the following cases, as judged from the intact gel electrophoresis: 1. After incubation of 1ug of lambda DNA-Hind III fragments with 300 units of enzyme for 24 hours at 37C 2. After incubation of 1ug of pBR322 DNA with 300 units of enzyme for 24 hours at 37C 3. Migrates as a single band of 50 kd in SDS-polyacrylamide gel electrophoresis. Note: Enzyme inhibits in a wide pH range, but most strongly at pH 7-8. Requires DTT of at least 1 mM to be active. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.