The ribosomal phosphoproteins(P protein antigens) are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35kD, P1 (19 kD) and P2 (17 kD). Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients. If anti-ribosomal P antibodies were to occur in the absence of other typical SLE associated autoantibodies, they may account for some patients with so-called ANA-negative lupus. It has been reported that lupus patients positive for anti-ribosomal P autoantibodies have a high frequency of CNS involvement, suggesting a marker use for these antibodies. Recombinant protein corresponding to human Ribosomal Phosphoprotein P0 expressed in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35,096 Dalton. RPLP0 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Immunological Functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels. Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & SLE sera (Systemic Lupus Erythematodes Disease). Coating Concentration: 0.3-0.7ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
