RIPA Lysis Buffer

Product Overview

• Article Name: RIPA Lysis Buffer
• Biozol Catalog Number: USB-R2031-75
• Supplier Catalog Number: R2031-75
• Alternative Catalog Number: USB-R2031-75-10,USB-R2031-75-25,USB-R2031-75-50,USB-R2031-75-100
• Manufacturer: US Biological
• Category: Biochemikalien
• Application: IP, WB

Product Description

RIPA lysis buffer is used to lyse cultured cells for immunoprecipitation and immunoblots. Similar in function to single detergent buffers that include NP-40, sodium chloride and Tris, RIPA is a good first choice for new antigens. Solubility: Colorless, clear, complete pH: 7.5 0.5 Components: 150mM NaCl 1.0% NP-40 0.5% deoxycholate (deoxycholic acid, sodium) 0.1% SDS 50mM Tris-Cl Molecular Biology Grade water 0.2um sterile-filtered Recommended Dilution: Immediately prior to use, add the protease and phosphatase inhibitors for the following final concentrations: 1mM PMSF, 1mM sodium orthovanadate, 1mM sodium fluoride, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin Storage and Stability: May be stored at 4C. Do not freeze. Stable for at 6 months after receipt. Store in the dark.