POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2), She
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2), She
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2), She
Biozol Catalog Number:
USB-R2039-20E
Supplier Catalog Number:
R2039-20E
Alternative Catalog Number:
USB-R2039-20E-100
Manufacturer:
US Biological
Host:
Sheep
Category:
Antikörper
Application:
WB
Immunogen:
E. coli-derived recombinant protein corresponding to Leu15-Leu354 of human RNA Polymerase II/POLR2A.
POLR2A (DNA-directed RNA Polymerase II subunit RPB1, also subunit A) is a 200-220kD member of the RNA polymerase beta-chain family of proteins. It is ubiquitously expressed and represents the largest subunit of RNA polymerase II, the catalytic mechanism for the synthesis of mRNA. Human POLR2A is 1970aa in length. It contains an N-terminus with six RNA polymerase domains (aa15-1079), and a C-terminus (CTD) with 52 seven aa repeats (-YSPTSPS-) that serve as a platform for polymerase subunit interaction. When these sequences are hyperphosphorylated, this subunit is called RNA pol IIo, when hypophosphorylated, the subunit becomes RNA pol IIa. Isoforms exist that show a 60aa substitution for aa1-95, a 24aa substitution for aa1-445, and an Arg substitution for aa1498-1536. Full-length human and mouse POLR2A sequences differ by only one amino acid (Thr1856Ala). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 0.02% NaN3. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
