The (Ro) SS-A antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases. The antigenic activity of the (Ro) SS-A antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA. Anti-(Ro) SS-A autoantibodies were described originally as precipitating autoantibodies in sera of Sjogrens Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the (Ro)SS-A antigen may be used as a diagnostic aid. Elevated levels of Anti-(Ro) SS-A have been detected in as high as 96% of Sjogrens Syndrome patients, 49% of patients with Systemic Lupus Erythematosus, 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-(Ro) SS-A and subdinical dry eyes or mouth was found in a substantial portion of an elderly population. In addition, it has been shown that 70% of patients with (Ro) SS-A precipitating antibodies also have rheumatoid factor. The titer of rheumatoid factor and the concentration of Anti-(Ro) SSA have been closely correlated in Primary Sjogrens Syndrome. Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers. Source: SS-A (RO) Antigen, from calf spleen. Unit Definition: One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for enzyme immunoassay. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight:
60
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in 0.01M Tris, 0.53M sodium chloride, 0.02% sodium azide.
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