Recombinant Rubella E1 envelope protein produced as a fusion protein in the eukaryotic expression system. Fusion protein MW: ~70kD. This protein binds to goat anti-Rubella polyclonal antibodies and Rubella converted human serum polyclonal antibodies in ELISA (IgG, IgM) and Western Blot. Recombinant protein corresponding to Rubella, E1 Envelope Protein, expressed in E. coli. Applications: Suitable for use in ELISA, Western Blot, Dot Blot, and Lateral Flow. Other applications not tested. Recommended Dilution: ELISA: 10-100ng depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized R9700-05P under standard ELISA conditions. Western Blot: 10-100ng depending on the nature and affinity of the detection reagent. Lateral Flow: 1-2mg/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
70
Purity:
95% (by SDS-PAGE, reduced).
Form:
Supplied as a liquid in PBS, pH 8.
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