SalI

Product Overview

• Article Name: SalI
• Biozol Catalog Number: USB-S0058
• Supplier Catalog Number: S0058
• Alternative Catalog Number: USB-S0058-2000,USB-S0058-5X1500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Sequence: 5-G T C G A C-3 3-C A G C T G-5 Source: Streptomyces albus Concentration: 10u/ul Unit Definition: One unit is defined as the amount of SaII required to digest 1ug of lambda DNA-Eco81I in fragments in 1 hour at 37C in 50ul of assay buffer. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Supplied with: R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37C. R1625-03: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM NaCl, 0.1mg/ml BSA. Incubate at 37C. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with SalI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with SalI, >95% of the DNA fragments can be ligated at a 5-termini concentration of 0.01uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of SaII for 4 hours. Blue/White Cloning Assay: pUC57 was incubated with 10 units of SalI for 16 hours. After religation and transformation, <1% of white colonies were detected. Methylation Effects: Dam, Dcm, EcoKI, EcoBI: never overlaps-no effect CpG: completely overlaps- blocked Star Activity: An excess of SalI (10u/ug DNA x 16 hours) may result in star activity. Stability during Prolonged Incubation: A minimum of 0.1 units of SalI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: SaII is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of SalI is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: Eco88I, SmlI, XhoI Number of Recognition Sites in DNA: Lambda: 2 PhiX174: 0 M13mp18/19: 1 pBR322: 1 pUC18/19: 1 pUC57: 1 pTZ19R/U: 1