The spike (S) glycoprotein of coronaviruses mediates viral entry into host cells. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. It is a type 1 viral fusion protein that characteristically contains two heptad repeat regions, denoted HR-N and HR-C, that form coiled-coil structures within the ectodomain of the protein. Previous studies have shown that the two heptad repeat regions can undergo a conformational change from their native state to a 6-helix bundle (trimer of dimers), which mediates fusion of viral and host cell membranes. SARS-ACSM contains the C-terminal t section of the Spike protein immunodominant fragments, amino acids: (1051-1076), (1121-1154), (1162-1190). Specificity: Immunoreactive with sera of SARS-infected individuals Application: Recombinant SARS-ACSM Antigen may be used in ELISA and Western Blot, excellent for detection of SARS with minimal specificity problems. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95%, Evaluated by SDS-PAGE.
Form:
Supplied as a liquid in 1.5M urea, 25mM Tris-HCl, pH 8.0, 0.2% Triton-X, 50% glycerol.
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