SdaI (Sse8387I)

Product Overview

• Article Name: SdaI (Sse8387I)
• Biozol Catalog Number: USB-S0549
• Supplier Catalog Number: S0549
• Alternative Catalog Number: USB-S0549-300,USB-S0549-1500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-C C T G C A G G-3 3-G G A C G T C C-5 E. coli that carries the cloned sdaIR gene from Streptomyces diastaticus Ng 7-324 Incubation Temperature: 37C Unit Definition: One unit is defined as the amount of Sdal required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of recommended reaction buffer. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate, pH 7.9 at 37C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA R1625-70: Restriction Enzyme Buffer for ScaI, SdaI, 10X: Dilute to 1X for use. 1X buffer composition is 37mM Tris-acetate, pH 7.0, 15mM magensium acetate, 150mM potassium acetate, 0.1mg/ml BSA Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 5-fold overdigestion with Sdal (5 units/ug lambda DNA x 1 hour) (see Star Activity). Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide occurredduring incubation with 10 units of Sdal for 4 hours. Blue/White (B/W) Cloning Assay: The B/W assay was replaced by the L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Enzyme Properties: Star Activity: An excess of enzyme (7.5u/ug DNA x 1 hour) may result in star activity. Methylation Effects: Dam/Dcm/CpG/EcoKl/EcoBl: never overlaps - no effect. Stability During Prolonged Incubation: A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: Alw21I, BseSI, Mph1103I, PstI, SduI Number of Recognition Sites in DNA: Lambda: 5 PhiX174: 0 pBR322: 0 pUC57: 0 pUC18/19: 1 pTZ19R/U: 1 M13mp18/19: 1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.