SduI

Product Overview

• Article Name: SduI
• Biozol Catalog Number: USB-S0577
• Supplier Catalog Number: S0577
• Alternative Catalog Number: USB-S0577-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

T T A A 5-G G G C C C-3 3-C C C G G G-5 T T A A Concentration: 10u/ul Source: Streptococcus durans RFL3 Supplied with: Catalog No: R1625-75: Restriction Enzyme Buffer for SduI, 10X 10mM Tris-HCl (pH 7.2), 3mM MgCl2, 150mM NaCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with SduI (see Star Activity). Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with SduI, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.4uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Star Activity: A large excess of enzyme (10u/ug DNA x 16 hours), low salt concentration or high pH may result in star activity. Stability during Prolonged Incubation: A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Compatible Ends: Alw21I, ApaI, Eco24I, Mph1103I, PstI, SacI, SdaI Number of Recognition Sites in DNA: Lambda: 38 PhiX174: 3 M13mp18/19: 5 pBR322: 10 pUC18/19: 5 pUC57: 6 pTZ19R/U: 5 pBluescriptIIKS(-/+): 6 pBluescriptIISK(-/+): 6 pACYC177: 4 pACYC184: 8 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.