Sex Hormone-Binding Globulin, Human, Western Blot Positive Control (SHBG, Sex Steroid-binding Protein, SBP, Testis-specific Androgen-binding Protein, BP, Testosterone-estradiol-binding Globulin, TeBG, Testosterone-estrogen-binding

Product Overview

• Article Name: Sex Hormone-Binding Globulin, Human, Western Blot Positive Control (SHBG, Sex Steroid-binding Protein, SBP, Testis-specific Androgen-binding Protein, BP, Testosterone-estradiol-binding Globulin, TeBG, Testosterone-estrogen-binding
• Biozol Catalog Number: USB-S1012-52G
• Supplier Catalog Number: S1012-52G
• Alternative Catalog Number: USB-S1012-52G-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: WB

Product Description

SHBG is a protein of mol wt 90kD (402aa) containing two subunits. SHBG transports the sex steroids testosterone, DHT and estradiol in the blood. Serum levels are increased during pregnancy, in women taking estrogens, and in men over the age of 50. Levels are reduced after menopause and in response to treatment with androgens or corticosteroids. Diagnostic uses include polycystic ovary disease and tests for liver and thyroid function Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. Source: Human SHBG, purified from pooled human serum. Molecular Weight: ~45kD Applications: Suitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested. Recommended Dilution: Western Blot: 10ul/lane SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 45
• Purity: ~90% (SDS-PAGE)
• Form: Supplied as a liquid in SDS-PAGE sample buffer (reduced).