SFRP1, Recombinant, Human (Secreted Frizzled Related Protein 1, SARP2, FrzA)

Product Overview

• Article Name: SFRP1, Recombinant, Human (Secreted Frizzled Related Protein 1, SARP2, FrzA)
• Biozol Catalog Number: USB-S1012-86R
• Supplier Catalog Number: S1012-86R
• Alternative Catalog Number: USB-S1012-86R-25
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Secreted Frizzled Related Proteins (sFRPs) are a family of secreted, soluble vertebrate glycoproteins which contain homology to the Wnt-binding domain of the Frizzled (Fz) family of transmembrane receptors. sFRPs are ~30-35kD in size and are comprised of 3 domains: a signal sequence, an N-terminal Fz cysteine-rich domain (CRD) with 10 conserved cysteines, and a C-terminal heparin-binding region with weak homology to Netrin. The Fz CRD mediates Wnt-binding and is present in all Fz and sFRP family members (1). sFRP-1, also known as secreted apoptosis-related protein 2 (SARP-2), FRP and FrzA, is expressed in the embryonic kidney, eye, brain, teeth, salivary gland and small intestine, most often at sites of epithelial-mesenchyme interaction (5). Expression in the adult animal is strong in the eye, kidney, and heart and also prevalent in the brain and lung (2,5). sFRP-1 was first characterized as a protein that enhances the sensitivity of cells to apoptotic stimuli (3) and as an antagonist of Wnt signaling in Xenopus embryos (4). It is also characterized as a tumor suppressor in breast (6) and cervical carcinomas (7). In contrast, sFRP-1 is expressed by the majority of malignant gliomas (8) and contributes to the development of uterine leiomyomas (9), suggesting that the role of sFRP-1 is dependent on cell context. sFRP-1 has diverse activities, from inducing angiogenesis (10) in a variety of in vivo models to helping regulate Wnt-4 signaling (with sFRP-2) in renal organogenesis (11). Mouse and human sFRP-1 proteins share 94% amino acid identity (1). Mouse myeloma cell line, NS0-derived human sFRP-1 protein Ser32-Lys314 with a C-terminal 6-His tag Molecular Mass: Mature recombinant human sFRP-1 is a monomeric protein. Based on N-terminal sequencing, three peptides, Ser31, Asp41 and Phe38 at their N-termini are present in this recombinant preparation in approximately equimolar amounts. As a result of glycosylation, the recombinant preparation migrates at ~35-40kD in SDS-PAGE under reducing conditions. Biological Activity: Measured by its ability to inhibit proliferation of human HeLa cervical adenicarcinoma cells. The ED50 for this effect is typically 2.5-10ug/ml. In an alternative assay, this protein also inhibits rmWnt-3A induced activation of beta-catenin reporter TOPFLASH in HEK293T cells. Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile PBS containing at least 0.1% HSA or BSA. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Purity: 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL).
• Form: Supplied as a lyophilized powder in PBS, pH 7.0, 50ug BSA/ug. Reconstitute with sterile PBS containing at least 0.1% HSA or BSA.