SHP 1, Recombinant, Human (SHPTP-1, SH2 containing protein tyrosine phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)
Biozol Catalog Number:
USB-S1013-25H
Supplier Catalog Number:
S1013-25H
Alternative Catalog Number:
USB-S1013-25H-10
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the ''motheaten and motheaten viable phenotypes that are severely autoimmune and immunodeficient.1 The enzyme is highly expressed in leukocyte cell types.2 SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors.3 Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity.4 SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor.5 A phosphopeptide containing the EGFR (Y992) sequence can be used to measure the activity of SHP-1 by detecting the release of phosphate. Source: E. coli-expressed recombinant protein. Definition: Measured by the ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site in 10mM HEPES, pH 7.5, 0.1mM EDTA, 0.1mM EGTA, 5 mg/mL BSA. Assayed for 30 minutes at 37 C. Specific Activity: 20umoles/min/mg. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Predicted 46kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
