Single-Stranded DNA Binding Protein, E. coli (SSB)

Product Overview

• Article Name: Single-Stranded DNA Binding Protein, E. coli (SSB)
• Biozol Catalog Number: USB-S1013-86R
• Supplier Catalog Number: S1013-86R
• Alternative Catalog Number: USB-S1013-86R-50,USB-S1013-86R-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kD subunits. It binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA. It is involved in DNA replication and in recombination in vivo. Spectral Ratio A280/A260: As Reported Binding Activity: ~5ug of SSB protein is required to prevent optical density change of 1ug of single-stranded DNA upon addition of 10mM MgCl2. Isoelectric Point: 6.0 Nucleotides Bound Per Monomer: 8-16 Thermostability: SSB retains activity after 20 min incubation at 65C. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Purity: 95% (SDS-PAGE)
• Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 200mM sodium chloride, 0.1mM EDTA, 1mM DTT, 50% glycerol