Smac, CT, Blocking Peptide (DIABLO, Direct IAP Binding Protein of Low Pi, Second Mitochondrial Activator of Cell Death)
Biozol Catalog Number:
USB-S1014-48P
Supplier Catalog Number:
S1014-48P
Alternative Catalog Number:
USB-S1014-48P-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, WB
The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2). Peptide (EERAESEQEAYLRED) corresponding to amino acids 225 to 239 of human Smac/DIABLO (1). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20C. May be stored at 4C for short-term only. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
* VAT and and shipping costs not included. Errors and price changes excepted
