SPHK1, Recombinant, Human, full length (Sphingosine kinase 1)

Product Overview

• Article Name: SPHK1, Recombinant, Human, full length (Sphingosine kinase 1)
• Biozol Catalog Number: USB-S5455-01
• Supplier Catalog Number: S5455-01
• Alternative Catalog Number: USB-S5455-01-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Human Sphingosine kinase 1 or SphK1 (GenBank Accession No. AF200328), full length with N-terminal His-tag, MW=46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D- erythro-sphingosine and [ -32P]ATP as substrates at pH 7.4 and 30 C. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay buffer: 50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5 mM ATP, uM D-erythro-sphingosine, and 0.5ug/ml sphingosine kinase 1. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 46.9
• NCBI: 33284
• UniProt: Q9NYA1
• Purity: 80% by SDS-PAGE
• Form: Supplied as a liquid in 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.