SspI

Product Overview

• Article Name: SspI
• Biozol Catalog Number: USB-S6600-90
• Supplier Catalog Number: S6600-90
• Alternative Catalog Number: USB-S6600-90-500,USB-S6600-90-2500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-A A T A T T-3 3-T T A T A A-5 Concentration: 10u/ul Source: Sphaerotilus species Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 15-fold overdigestion (15u/ug lambda DNA x 1 hour) with SspI (see Star Activity). Ligation/Recutting Assay: After 10-fold overdigestion (2u/ug DNA x 5 hours) with SspI, more than 90% of the DNA fragments can be ligated at a 5-termini concentration of 0.3uM. More than 90% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Star Activity: An excess of enzyme (20u/ug DNA x 1 hour) may result in star activity. Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Number of Recognition Sites in DNA: Lambda: 20 PhiX174: 1 M13mp18/19: 6 pBR322: 1 pUC18/19: 1 pUC57: 1 pTZ19R/U: 2 pBluescriptIIKS(-/+): 2 pBluescriptIISK(-/+): 2 pACYC177: 2 pACYC184: 2