Stem Cell Growth Factor beta, Recombinant, Human (SCGFb)

Product Overview

• Article Name: Stem Cell Growth Factor beta, Recombinant, Human (SCGFb)
• Biozol Catalog Number: USB-S7973-30
• Supplier Catalog Number: S7973-30
• Alternative Catalog Number: USB-S7973-30-2,USB-S7973-30-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Stem Cell Growth Factor alpha and beta are hematopoietic growth factors that exert their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGFb is a 25kD polypeptide containing 227 amino acid residues. Endotoxin: 0.1ng/ug (1EU/ug). Biological Activity: Determined by the dose dependant stimulation of the proliferation of human TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed is 7ng/ml. Amino Acid Sequence: MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Add carrier protein and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA

Product Properties

• Molecular Weight: 25
• Purity: 98% by SDS-PAGE and HPLC.
• Form: Supplied as a lyophilized powder from PBS, 1mM DTT. Reconstitute with sterile ddH2O to 0.1-1.0mg/ml. DO NOT VORTEX. Add buffer containing carrier protein for long term storage.