Horseradish Peroxidase conjugated Streptavidin is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears. In addition, sensitive detection systems for a variety of solid-phase assays utilizing microtitration plates or nitrocellulose have been developed using this conjugate. Horseradish Peroxidase Streptavidin is supplied as a concentrate (1mg/ml) or as a ready-to-use (R.T.U.) stabilized solution (100 ml, 1ug/ml) in a bottle fitted with a drop dispenser top. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Moles of HRP/Moles of Streptavidin: ~1.5 Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Form:
Supplied as a liquid. Labeled with Horseradish peroxidase (HRP).
CAS Number:
[9013-20-1]
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