Applications: Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Absorption Wavelength: 581nm Emission Wavelength: 596nm Fluorochrome/Protein Ratio: 1.9 molesCy3.5 per mole of Streptavidin Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified from chromatographically pure streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-streptavidin
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with Cy3.5.
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