Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10-e15M). It is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding resulting in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background. Applicaitons: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Recommended for use in detection systems utilizing biotinylated antibodies and other biotinylated molecules. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:150-1:500 Western Blot (chromagenic): 1:1250-1:5000 Western Blot (chemiluminescence): 1:2500-1:10,000 ELISA: 1:2500-1:5000 Optimal dilutions to be determined by the researcher.. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight:
66
Purity:
Purified
Form:
Supplied as a liquid in 0.1% Proclin, glycerol. Labeled with horseradish peroxidase (HRP).
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