Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10e-15M). It is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding resulting in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:20-1:50 Flow Cytometry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months after receipt at 4C. Freezing CY5 conjugates will result in a substantial loss of activity. CY5 conjugates are sensitive to light.
Purity:
Purified
Form:
Supplied as a liquid in 0.09% sodium azide. Labeled with CY5.
* VAT and and shipping costs not included. Errors and price changes excepted
