Synthetic peptide corresponding to the C-terminal cytoplasmic part of STIM1
STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension. Applications: Suitable for use in Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using RBL rat basophilic leukemia cell line under both reducing and non-reducing conditions. Immunohistochemistry (paraffin): 5ug/ml Immunocytochemistry: Methanol-aceton fixation Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
