Succinimidyl-6-(biotinamido) Hexanoate is a biotinylation reagent incorporating an aminocaproyl ''spacer. This can reduce the steric hindrance in binding avidin to some biotinylated compounds when used to biotinylate both the antibody and the carrier in the Protein Avidin-Biotin Capture System. This system avoids the direct interaction of a captured antibody with solid surfaces, such as plastics, which may reduce antigenicity. It has also been used to enhance the detection of DNA on nitrocellulose. Has also been used as a cell surface labelling reagent Melting Point: 150-164C Solubility: DMF, DMSO, Methanol (Sparingly) Method for Determining Identity: Proton NMR Spectroscopic and Mass Spectrometric Analysis TLC Conditions: SiO2, Dichloromethane: Methanol: 9:1, Visualized with AMCS, Single spot Rf=0.6. Storage and Stability: May be stored at RT for short-term only. Long-term storage is recommended at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight:
454.55
Purity:
~90%
Form:
White to Off-White Solid
CAS Number:
[72040-63-2]
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