SUMO-activating Enzyme Subunit 2, CT (E616) (Ubiquitin-like 1 Activating Enzyme E1B, Anthracycline-associated Resistance ARX, HRIHFB2115, SAE2, UBA2, UBLE1B), Rabbit
Catalog Number:
USB-S8025-90D
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| Article Name: | SUMO-activating Enzyme Subunit 2, CT (E616) (Ubiquitin-like 1 Activating Enzyme E1B, Anthracycline-associated Resistance ARX, HRIHFB2115, SAE2, UBA2, UBLE1B), Rabbit |
| Biozol Catalog Number: | USB-S8025-90D |
| Supplier Catalog Number: | S8025-90D |
| Alternative Catalog Number: | USB-S8025-90D-200 |
| Manufacturer: | US Biological |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC, WB |
| Immunogen: | Synthetic peptide selected from the C-terminal region of human UBA2 (KLH). |
| Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442aa UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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