A deletion mutant of alpha synuclein that lacks the NAC region(amino acid 61-95). Alpha synuclein (delta NAC) was overexpressed in E. coli. 6 amino acids were added as a linker. Molecular Weight: 11,935 (111 amino acids, Real molecular size on SDS-PAGE will be shift up). Sequence: MDVFMKGLSK/ AKEGVVAAAE/ KTKQGVAEAA/ GKTKEGVLYV/ GSKTKEGVVH/ GVATVAEKTK/ GTEIWMKKDQ/ LGKNEEGAPQ/ EGILEDMPVD/ PDNEAYEMPS/ EEGYQDYEPE/ A Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
11935
Purity:
95% by SDS PAGE, Purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques.
Form:
Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride.
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