TaaI (Tsp4CI)

Product Overview

• Article Name: TaaI (Tsp4CI)
• Biozol Catalog Number: USB-T0290
• Supplier Catalog Number: T0290
• Alternative Catalog Number: USB-T0290-200,USB-T0290-1000
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-A C N G T-3 3-T G N C A-5 Unit Definition: One unit is defined as the amount of Taal required to digest 1ug of lambda DNA in 1 hour at 65C in 50ul of reaction buffer. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Storage Buffer: 10mM Tris-HCl, pH 7.5, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Methylation Effects: Dam: never overlaps - no effect Dcm: never overlaps - no effect CpG: may overlap - cleavage impaired EcoKl: may overlap - effect not determined EcoBl: may overlap - effect not determined Number of Recognition Sites in DNA: Lambda: 187, PhiX174: 15 pBR322: 14, pUC57: 8, pUC18/19: 8, pTZ19R/U: 7, M13mp18/19: 31 Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 65C. Compatible Ends: Hpy188l Incubation Temperature: 65C Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20 minutes. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with TaaI. Ligation/Recleavage Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a facrtor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide was observed after incubation with 10units of Taal for 4 hours. Storage and Stability: Store at -20C. Aliquots are stable for 6 months after receipt.