TAB182, Human (Tankyrase-binding protein of 182kD) (Control Peptide)
Biozol Catalog Number:
USB-T0292-02
Supplier Catalog Number:
T0292-02
Alternative Catalog Number:
USB-T0292-02-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, WB
A 15-aa Peptide (designated as TAB182-P, Control peptide) ~ within the C-temrinus of human/mouse TAB182. The control peptide sequence is 100% conserved in mouse and human Tab182. Tankyrases (TRF1 interacting ankyrin-related ADP-ribose polymerase, human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8, and its homolog TANK2), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. In addition to regulating the telomere and nuclear localization, TANKs also resides at the other subcellular compartments such as nuclear envelope, specifically on cytoplasmic fibers of nuclear pore complexes. TANk1 is targeted to pericentriolar domain of centrosomes during mitosis. After mitosis, TANK1 associates with the golgi as a peripheral membrane protein and implicated in targeting of GLUT4 vesicles. In the absence of insulin, GLUT4 vesicles reside in the Golgi and cytoplasm. Upon stimulation by insulin, GLUT4 vesicles undergo exocytosis and translocate to the plasma membrane. GLUT4 vesicles are characterized by the presence of glucose transporter 4 (GLUT4) and IRAP (Insulin regulated aminopeptidase). The exocytosis of GLUT4 vesicles is therefore plays an important role in uptake of glucose through Glut4 and the degradation of various vascoactive hormones by IRAP. TANKs have been colocalized in GLUT4 vesicles and shown to bind IRAP via the ANK-repeats. Recently a novel TANK1-binding protein termed TAB182 (tankyrase-binding protein of 182kD), which binds to the ANK domain of TANK1 and TANK2 has been identified. TAB182 (human 1729 aa, predicted size 182kD, actual size ~200kD) is expressed in heart, lung, liver, kidney, pancreas, testing, ovary etc. TAB182 localizes to nucleus and to the cytoplasm, where it co localizes with cortical actin network. It serves as an acceptor of poly(ADP-ribosyl)ation by TANK1. TANK1 is suggested to act as scaffold for large molecular mass complexes made up of multiple binding proteins. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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