Telomerase Protein, Mouse, Control Peptide (Tep1, MGC91078, p240, p80 telomerase homolog, Telomerase-associated protein 1, Telomerase protein 1, Telomerase protein component 1, Tp1)
Biozol Catalog Number:
USB-T2399-65
Supplier Catalog Number:
T2399-65
Alternative Catalog Number:
USB-T2399-65-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, WB
The 3ends of chromosomes are capped with telomere sequences (TTAGGG, 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities. Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity) and show 20% homology. Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2, hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned. Est2 is associated with telomerase activity and TP1. Mouse/rat and human TP1 are 2627 and 2629 aa proteins, respectively. A 19aa peptide sequence in the mid-region of mouse TP1. Uniprot/Accession: P97499 Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot due to low MW. Other applications not tested. Recommended Dilutions: ELISA: 1ug/ml to coat plates. Antibody Blocking: 5-10ug peptide. Optimal dilution determined by the researcher. Species Sequence Homology: rat, 100%, human, 89% Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with 100ul sterile PBS.
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