Terminal Deoxynucleotidyl Transferase (TdT)

Product Overview

• Article Name: Terminal Deoxynucleotidyl Transferase (TdT)
• Biozol Catalog Number: USB-T2750
• Supplier Catalog Number: T2750
• Alternative Catalog Number: USB-T2750-500,USB-T2750-2500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

TdT is a template-independent DNA polymerase located in the cell nucleus which catalyses irreversible terminal addition, via polymerization, of deoxynucleotides at the 3 hydroxyl ends of oligo or polydeoxynucleotide initiators accompanied by the release of inorganic phosphate. Applications: Oligo and DNA 3-terminal labeling Homopolymeric tailing of linear duplex DNA with any type of termini TUNEL assay (in situ localization of apoptosis) 5-RACE Components: Terminal Deoxynucleotidyl Transferase: 1x500U or 1x2500U Reaction Buffer (5X): 1x0.4ml or 2x1ml 1M potassium cacodylate, pH 7.2 (25C), 5mM CoCl2, 0.5mM DTT, 0.05% Triton X-100 Storage Buffer: 100mM potassium acetate, pH 6.8, 2mM 2-mercaptoethanol, 0.01% Triton X-100, 50% glycerol. Unit Definition: 1 unit of the enzyme iincorporates 1 nanomole of deoxythymidylate into a polynucleotide fraction in 60 minutes at 37C. Note: Due to the presence of CoCl2, the TdT Reaction Buffer is incompatible with downstream applications. It is necessary to remove CoCl2 from the reaction mixture by spin column or phenol/chloroform extraction and subsequent ethanol precipitation. Quality Control: Tested for absence of exodeoxyribonucleases, endodeoxyribonucleases ribonucleases, and phosphatases. Inhibitors: Metal chelators, ammonium chloride, iodide and phosphate ions. Inactivators: Inactivated by heating at 70C for 10 mins. or by addition of EDTA. Storage and Stability: Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.