Thialysine (S-Aminoethyl-L-Cysteine Hydrochloride) 98+%, CAS [[4099-35-8]]
Biozol Catalog Number:
USB-T5000
Supplier Catalog Number:
T5000
Alternative Catalog Number:
USB-T5000-1,USB-T5000-5
Manufacturer:
US Biological
Category:
Biochemikalien
The lysine analog, thialysine, has been implicated in the mechanism for the inhibitory effect of the on human acute leukemia Jurkat T cells. When Jurkat T cells were treated with thialysine (0.32-2.5mM), apoptotic cell death along with several biochemical events such as mitochondrial cytochrome c release, caspase-9 activation, caspase-3 activation, degradation of poly (ADP-ribose) polymerase, and DNA fragmentation was induced in a dose- and time-dependent manner. However, these thialysine-induced apoptotic events were significantly abrogated by an ectopic expression of Bcl-xL, which is known to block mitochondrial cytochrome c release. Decylubiquinone, a mitochondrial permeability transition pore inhibitor, also suppressed thialysine-induced apoptotic events. Comparison of the thialysine-induced alterations in the cell cycle distribution between Jurkat T cells transfected with Bcl-xL gene (J/Bcl-xL) and Jurkat T cells transfected with vector (J/Neo) revealed that the apoptotic cells were mainly derived from the cells accumulated in S and G2/M phases following thialysine treatment. The interruption of cell cycle progression in the presence of thialysine was accompanied by a significant decline in the protein level of cdk4, cdk6, cdc2, cyclin A, cyclin B1, and cyclin E. These results demonstrate that the cytotoxic activity of thialysine toward Jurkat T cells is attributable to not only apoptotic cell death mediated by a mitochondria-dependent death signaling pathway, but also interruption of cell cycle progression by a massive down-regulation in the level of cdks and cyclins. Synonyms: S-(2-Aminoethyl)-L-cysteine Monohydrochloride, NSC 186915, S-(2-Aminoethyl)-L-cysteine Hydrochloride, S-(2-Aminoethyl)-L-cysteine Monohydrochloride, S-(beta-Aminoethyl)-L-cysteine Hydrochloride, Thialysine Hydrochloride CAS No: 4099-35-8 Molecular Formula: C5H12N2O2SHCl Molecular Weight: 200.70 Purity (TLC): 99% Appearance: Light beige powder Melting Point: 200-202C Specific Rotation [a]D24: [a]D24 = -6.5 1 (C=1 in Water) Chloride: 18% (AgNO3) Solubility (H2O, 50mg/ml): Light tan, clear Storage and Stability: Store at 4C. Stable for 12 months after receipt.
Molecular Weight:
200.7
CAS Number:
[4099-35-8]
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