Thrombomodulin, Recombinant, Human (CD141, TM, Fetomodulin)

Product Overview

• Article Name: Thrombomodulin, Recombinant, Human (CD141, TM, Fetomodulin)
• Biozol Catalog Number: USB-T5048-02
• Supplier Catalog Number: T5048-02
• Alternative Catalog Number: USB-T5048-02-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Encoded by the THBD gene, thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of human THBD (Accession NP_000352) predicts a signal peptide (aa 1-18) and a mature chain (aa 19-575) that consists of following domains: C-type lectin (aa 35-169), EGF-like (aa 242-281, aa 284-324, aa 325-363, aa 365-405, aa 404-440, and aa 441-481), Ser/Thr-rich (aa 490-506), transmembrane (aa 516 to 539) and cytoplasmic (aa 540-575) (1). rhTHBD consists of aa 19-515, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (1-3). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (4). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (5). Mouse myeloma cell line, NS0-expressed recombinant protein corresponding to human Thrombomodulin/BDCA-3 proteinm Ala19-Ser515 (Ala473Val), with a C-terminal 6-His tag Endotoxin Level: < 1.0 EU per 1 µg of the protein as determined by the LAL method. Activity: Measured by its ability to mediate thrombin activation of protein C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• NCBI: 000352
• UniProt: P07204
• Purity: 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form: Supplied as a liquid in 25mM Tris, 0.15M sodium chloride, pH 7.5.