Human Tie2 GenBank Accession No. NM_000459), (a.a. 771-end) with N-terminal His tag, MW 45kD, expressed in an Baculovirus infected Sf9 cell expression system. Specific Activity: 140 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30 C. Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na orthovanadate, 0.5mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 0.2ug recombinant Tie2. Application: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
