Tissue Factor, Western Blot Positive Control (TIF)
Biozol Catalog Number:
USB-T5575-03B
Supplier Catalog Number:
T5575-03B
Alternative Catalog Number:
USB-T5575-03B-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
Tissue factor (TF) is the membrane receptor for coagulation factors VII and VIIa and is a cell-surface initiator of coagulation. It is the major molecule of this type and is critical for controlling hemostasis, thrombosis, and inflammation. TF is not generally present in or on cells within the vasculature, but following vascular injury, blood can come into contact with TF expressed by extravascular cells, resulting in activation of the coagulation cascade. Source: Human Tissue factor, expressed in Sf9 cells. Applications: Suitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required. Other applications not tested. Recommended Dilution: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly Purified (~95%)
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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