Tissue, Total Protein, Matched Pairs, Human Primary Tumor and Metastasis Tumor, Lung HisTek(TM)
Biozol Catalog Number:
USB-T5595-6921M
Supplier Catalog Number:
T5595-6921M
Alternative Catalog Number:
USB-T5595-6921M-2X200
Manufacturer:
US Biological
Category:
Sonstiges
Description: All the protein lysates were prepared from liquid nitrogen fresh frozen tissues. Total protein and universal protein lysates were prepared by a Total Protein Extraction Kit (Cat P9087). Human tumor protein matched products include: Primary Pair (PP), or Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue, PM consists of total protein from primary tumor and corresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific genes and tumor metastatic genes Quality Control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining, the pattern is consistent with each lot. 2. The isolated protein is analyzed by Western Blot using an antibody to GAPDH for total protein, cytoplasmic and universal total protein. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for at least 6 months. After the protein is thawed, store it at 4C for continuous use. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form:
Supplied in a buffer including HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
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