| BioGenomics(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection. BioGenomics(TM) Universal cDNA, Mixed Male and Female donors. Prepared by reverse transcribed RNA by using random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes. Applications: Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction, Profiling study in gene expression. Other applications not tested. Species Selection: Choose from Human, Mouse, Rat, Chicken, Canine, Monkey. Human Universal cDNA is prepared from major organs of both male and female adult donors Mouse Universal cDNA is prepared from several male and female Balb/C mice whole bodies without fur. Rat Universal cDNA is prepared from several male and female Sprague Dawley or Wistar rats whole bodies without fur. Chicken (Leghorn) Universal cDNA is prepared from major organs of both male and female adult donors. Canine (Beagle) Universal cDNA is prepared from major organs of both male and female adult donors Monkey (Cynomolgus and Rhesus) Universal cDNA is prepared from major organs of both male and female adult donors Supplied With: T5595-0010: Tissue, cDNA, Actin Control Primer Concentration: ~2.5ng/ul, 1ul cDNA is sufficient for one PCR reaction. Control PCR Component Is As Follows: Taq polymerase 0.2ul 10X PCR buffer 2.5ul 10mM dNTP 0.5ul Control primers (5uM) 1.0ul PCR Ready First Strand cDNA 1.0ul Sterile ddH2O 19.8ul Total Volume 25.0ull Control PCR Conditions: 1. 94C x 2 minutes, 1 cycle. 2. 94C x 30 seconds, 55C x 30 seconds, 72C x 30 seconds, 35 cycles. 3. 72C x 5 minutes, 1 cycle. Hold at 4C. Quality Control: 1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). The ratio of 28S/18S is 1. 2. The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR. 3. The synthesized human, animal, and cell line cDNA was 5 selected to ensure its full length. The cDNA was used as template for PCR amplification of beta-actin gene and an 838 bp beta-actin band was visualized on 1% agarose gel. beta-actin control primer is included. It is enough for 10 PCR reactions. 4. The synthesized plant cDNA was used as template for PCR amplification of chloroplast gene. A 458 bp chloroplast band was visualized on 1% agarose gel. Chloroplast control primer is included. It is enough for 10 PCR reactions. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
