Beta-TrCP plays a relevant role in the control of stability of several key protein factors. In Xenopus, beta-TrCP acts as an inhibitor of Wnt signaling and dorsal axis formation. A variety of cellular stress can induce the level of beta-TrCP mRNA and protein levels in human cells. Activation of stress-activated protein kinases JNK by forced expression of constitutively active mutants of JNKK2 and MKK6 also leads to accumulation of beta-TrCP. Another role played by beta-TrCP is the targeting of Cdc25A for degradation by the Skp1/Cul1/F-box protein complex. Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[6A200]
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Horseradish peroxidase (HRP).
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